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double-stranded dna中文是什么意思

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用"double-stranded dna"造句"double-stranded dna"怎么读"double-stranded dna" in a sentence

中文翻译手机手机版

  • 双链dna

例句与用法

  • The cyanophage contained a double - stranded dna genome at ca . 36 . 6kb
    病毒的遗传物质为双链dna ，基因组的大小约36 . 6kb 。
  • The genetic material of the new virus is arranged in a similar fashion , encoded in circular , double - stranded dna , and the virus ' s five proteins have similarities to the proteins of other polyoma viruses
    新病毒的遗传物质排列方式与多瘤病毒类似，编码为环状的双链dna ，新病毒的五个蛋白质与其他多瘤病毒有相似之处。
  • To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
    方法：根据端粒酶htert基因1573 ? 1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna 。
  • Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
    结论：以带t7启动子部分双链dna为模板，用t7rna聚合酶体外合成出的shrna产量较高，纯度较好，是一种简便、高效、低成本的短链rna的制备方法，适合于普通实验室用来进行短链rna的合成和rna干扰实验。
  • The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
    本研究设计以c tamrna为模板的反义cdna片段，从c ta基因5 ’端第94位到500位核苷酸段设计引物，目的片段407bp ，覆盖第116和188位两个aug密码子，也包含了第一外显子和第二外显子间的剪接位点：用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ；腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒，用氯化铯密度梯度离心法获得纯化的高滴度腺病毒；进行体外基因转移，分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞，观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用，从而达到调控mhc -类分子表达的目的。
  • In this study , the suitable parameters for the introduction of plasmids phz1358 and pset152 into s . nanchangensis ns3226 from e . coli were tested for developing an conjugation system for s . nanchangensis ns3226 . a dnd gene cluster , which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e . coli into s . nanchangensis ns3226
    将克隆在整合型载体pset152上的变铅青链霉菌1326的dnd基因簇通过接合转移导入野生型南昌链霉菌ns3226中进行异源表达，观察到接合转移子的dna获得了在含fe ~ ( 2 + )的电泳缓冲液中电泳时降解的表型。
用"double-stranded dna"造句  

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